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Image Search Results
Journal: Nature Communications
Article Title: Surfaceome dynamics reveal proteostasis-independent reorganization of neuronal surface proteins during development and synaptic plasticity
doi: 10.1038/s41467-020-18494-6
Figure Lengend Snippet: a cLTP paradigm b The plot shows averaged fast EPSC amplitudes for each cell (triangles) and their averages (mean ± s.e.m) for control (untreated, n = 45) and cLTP (glycine treated, n = 38) cells. Mann–Whitney test. c – f Boxplots of distributions of log2 surface abundances for replicates of cLTP ( n = 12) and control ( n = 12). Boxes indicate median and percentiles (25th and 75th). g Volcano plot of statistical significance ( y -axis) and surface abundance change ( x -axis). Horizontal line is located at an adjusted p -value of 0.05. h Proteins with significantly different surface abundances (fold-change > 1.5 and p < 0.05) after cLTP grouped into categories. Shape size indicates scaled −log10 adjusted p -value. Border color indicates fold-change directionality (green > 0, purple < 0). Edges represent string confidence > 0.7. i Representative images of analysed primary dendrites before and after cLTP induction. Bars 5 µm. j Quantification of mean fluorescent intensity of antibody signal on the primary dendrite surface using antibodies against GluA: n = 63, Bai1: n = 42, Adcy3: n = 28 (CTRL) n = 22 (cLTP) cells/group. Means + 95% CI presented. Two-way T -test (Adcy3 + Bai1) or Mann–Whitney test (GluA). * p < 0.05, ** p < 0.01, **** p < 0.0001. Source data are provided as a Source Data file.
Article Snippet: Antibodies targeting the extracellular domains of
Techniques: MANN-WHITNEY